Journal: Nature Communications

Article Title: Collagen binding adhesin restricts Staphylococcus aureus skin infection

doi: 10.1038/s41467-026-68788-4

Figure Lengend Snippet: a Multispectral quantitative pathology of skin abscess tissue excised from mice infected with WT, Δ cna or comp cna bacteria 3 days post inoculation and stained with antibodies targeting 6 host proteins as described in the legend. Images depict staining of entire tissue section as used for quantitative analysis. b Images digitally zoomed in (X6.5) from sections shown in ( a ). Regions of interest are depicted in a as magenta boxes. Images demonstrate staining from tissues infected with WT ( b ), Δ cna ( c ) or comp cna ( d ) and stained with antibodies targeting 6 host proteins as shown in legend. Images are separated according to channels that demonstrate spatial distribution of bacteria with collagen (DAPI, Collagen), CD45 cells, neutrophils (CD45, Ly6G) and macrophages (CD45, F480). e Quantification of staining demonstrated in ( a ) represented as total cells per millimeter of tissue sections infected with strains as described above. Staining of tissue was repeated for a total n = 2.

Article Snippet: Imaging shown includes antibodies against CD11b (1:1000 Novus, Cat No: NB110-89474), Ly6G (1:100 Cell Signaling Tech, Cat No: 87048S), COL1A1 (1:100 Cell Signaling Tech, Cat No: 72026S), CD45 (1:100 Cell Signaling Tech, Cat No: 70257S), F480 (1:100 Cell Signaling Tech, Cat No: 30325S) , .

Techniques: Infection, Bacteria, Staining